Krios 5 Cryo-TEM Features

Cryo-transmission electron microscope for high-fidelity atomic resolution cryo-EM

The 300 kV Thermo Scientific Krios 5 Cryo-Transmission Electron Microscope (Cryo-TEM) has been designed for high-performance single particle analysis (SPA), cellular cryo-electron tomography (cryo-ET) and microcrystal electron diffraction (MicroED). The Krios 5 Cryo-TEM is capable of quickly producing high-quality structures thanks to a unique focus on high throughput with high data fidelity, increased automation of routine alignments, improved optical precision, and AI-enabled software, which make it a highly productive and “ready-to-use” system for all expertise levels across multiple scientific fields.

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Take a tour of the Krios 5 Cryo-TEM

The Thermo Scientific Krios 5 Cryo-TEM rapidly produces high-quality structures with a focus on data fidelity. Enhanced automation, improved optical precision, and AI-assistance make it productive and user-friendly. Its compact, sustainable design includes Eco modes to reduce energy consumption.

Experience up to 25% faster throughput relative to previous Krios Cryo-TEM generations with high data fidelity for single particle analysis. Extended aberration-free image shift (AFIS) accelerates data collection, while AI-powered Thermo Scientific Smart EPU Software plugins automate routine alignments. Thermo Scientific Tomography 5 Software provides high image quality via automatic 8K Electron Event Representation (EER) decoding and on-the-fly motion correction for crisp tomograms, unlocking the full potential of the Thermo Scientific Falcon 4i Direct Electron Detector beyond the Nyquist limit. Easily transfer Autogrid cassettes between Autoloaders contamination-free with the Thermo Scientific Vacuum Capsule. Designed for sustainability, the Krios 5 Cryo-TEM features energy-saving Eco modes and environmental footprint traceability with a My Green Lab ACT Ecolabel.

Krios 5 Cryo-TEM benefits for cell and structural biology research

High-data-fidelity atomic-resolution cryo-EM

The Krios 5 Cryo-TEM offers up to 25% faster throughput with high data fidelity, extended aberration-free image shift (AFIS) and magnification calibration accuracy within 1% for single particle analysis. Combined with the optional Thermo Scientific Selectris X Imaging Filter and E-CFEG, the Krios 5 Cryo-TEM can deliver enhanced image contrast for rapid, atomic-resolution imaging.

Faster single particle analysis with AI-automation

AI-assistance with Smart EPU Software enables automated grid screening, quality monitoring, and data management. Smart Plugins in the Smart EPU Software use AI-based image analysis to automate grid area selection and dynamically adjust settings based on real-time analysis.

Accelerated, contamination-free cryo-electron tomography workflow

Tomography 5 Software provides automatic 8K Electron Event Representation (EER) decoding and on-the-fly motion correction for crisp tomograms by unlocking the full data potential beyond the Nyquist limit. Manage tomograms and experimental parameters with the Discovery Viewer in Thermo Scientific CryoFlow Software.  Easily transfer Autogrid cassettes between Autoloaders contamination-free with the Vacuum Capsule.

Energy-saving Eco modes and My Green Lab ACT Ecolabel

The Krios 5 Cryo-TEM features energy-saving Eco modes with fast “wake up” scheduling, plus environmental impact certification with the My Green Lab® ACT® Ecolabel

Learn more about cryo-electron microscopy with the Krios 5 Cryo-TEM

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Explore the structure of life with the Krios 5 Cryo-TEM

The Thermo Scientific Krios 5 Cryo-TEM rapidly produces high-quality structures with a focus on data fidelity. Enhanced automation, improved optical precision, and AI-assistance make the instrument productive and user-friendly.
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Revolutionizing Cryo-Tomography: Introducing the Vacuum Capsule for Contamination-Free Transfers

Explore the new Thermo Scientific Vacuum Capsule, designed for contamination-free sample transfer. Learn how it improves cryo-tomography lamellae production and enables the safe transfer of biological materials that require certain levels of biosafety.

Unlock data beyond the Nyquist frequency and accelerate your cryo-ET workflow

Tomography 5 Software provides 8k electron-event representation decoding and on-the-fly motion correction, automatically capturing data beyond the Nyquist frequency for crisp tomograms and the high image quality required for sub-tomogram averaging. 

Chlamydomonas reinhardtii 80S ribosome protein structure determined with Krios Cryo TEM

AI-assistance with SmartEPU Software

AI-powered SmartEPU plugins automate data acquisition by analyzing images in real-time. They can be used to select optimal grid squares, adjust focus, classify foil holes, predict ice thickness, and skip poor-quality areas, significantly enhancing data collection efficiency in single particle analysis.

Preview of Smart EPU Software, used to select foil holes for cryo EM imaging

Automation of daily alignments

Routine alignments, including beam and aperture centering as well as coma and astigmatism correction, are fully automated. Magnification calibration within 1% and a diagnostics dashboard ensure optimal maintenance.

Preview of optics alignment in Thermo Scientific Microscope Companion Software for cryo EM

Low-power Eco modes

The low-power Eco modes of the Krios 5 Cryo-TEM deactivate specific parts of the instrument when it is not in use, reducing power consumption up to 80%. The software can be programmed for early wake-up, ensuring the system is ready when needed.

Front facing image of Krios 5 Cryo-TEM in Eco mode

Designed-in sustainability with the ACT Ecolabel

In addition to the energy-saving Eco modes of the Krios 5 Cryo-TEM, the My Green Lab ACT Ecolabel transparently documents the instrument’s environmental footprint, including manufacturing, energy and water use, as well as end-of-life disposal. At Thermo Fisher Scientific, our commitment to sustainability supports our mission to make the world healthier, cleaner, and safer.

ACT label logo

Krios 5 Cryo-TEM imaging capabilities

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Extended aberration-free image shift

Aberration-free image shift is an optical mode for fast data acquisition that does not compromise image quality. It can perform large beam shifts to an extended range of 20 micrometers on the Krios 5 Cryo-TEM without off-axis coma and astigmatism.
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Fringe-free imaging with Dr. Elizabeth Villla

Acquire more images per sample area with fringe-free imaging, enhancing your daily data throughput. Hear how fringe-free imaging has been helping the daily research in the lab of Dr. Elizabeth Villa.
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Electron event representation and the Falcon 4i Direct Electron Detector

The Falcon 4i Direct Electron Detector combines high image quality, high throughput, and efficient lossless data compression via electron-event representation. Tomography 5 Software offers on-the-fly motion correction and access to electron-event-representation information beyond the Nyquist frequency for high-quality sub-tomogram averaging.

Upgrade options for previous-generation Krios Cryo-TEMs

Electron source: cold field emission gun (E-CFEG)

 

E-CFEG is a low-energy spread cold field emission gun for higher contrast at truly atomic resolution. Low energy spread (≤0.3 eV) enables higher contrast at high resolutions (≤2.0 Å); Improved contrast at high spatial frequencies to reach higher resolutions in less time.

Comparison of field emission gun contrast on Krios Cryo TEM for atomic resolution cryo EM Comparison of a Thermo Scientific high-brightness field emission gun and the cold field emission gun on the Krios Cryo-TEM.

Krios Cryo-TEM accessories and software

 

Increase your lab’s productivity and performance with innovative upgrades:

Falcon 4i Direct Electron Detector

Cryo-EM research in BSL-3 containment facilities

Work on contagious pathogens frequently requires specialized biosafety containment facilities. Until recently, cryo-EM equipment was not commonly found in these laboratories. At Thermo Fisher Scientific, we have developed products and services designed for these higher BSL facilities, so that our customers can accelerate their critical pathogen research with cryo-electron microscopy.

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Introducing the 60°C heat decontamination for Krios Cryo-TEM for biosafety labs

This solution enables installation and service support of Krios Cryo-TEMs and Thermo Scientific Arctis Cryo-Plasma-FIBs in higher biosafety-level containment facilities (e.g., BSL-3). Additionally, the Vacuum Capsule securely contains samples, allowing for the safe transfer of biological materials that require certain levels of biosafety.
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BSL-3 cryo-electron tomography study of SARS-CoV-2 at Hokkaido University

Researchers at Hokkaido University have been using a Krios G4 Cryo-TEM in BSL-3 conditions to study structures of pathogens like SARS-CoV-2 to support vaccine development.

Cryo-EM in Biosafety Facilities for Virology Research

Cryo-EM in Biosafety Facilities for Virology Research

Krios Cryo-TEM technical specifications

Krios Cryo TEM Datasheet

Preview of Krios 5 Cryo-TEM datasheet

Selectris Imaging Filters

Preview of Selectris Imaging Filter datasheet

Falcon 4i Direct Electron Detector

Preview of Falcon 4i datasheet

Vacuum Capsule datasheet

Preview of Vacuum Capsule datasheet

Smart EPU Software datasheet

Preview of Smart EPU Software datasheet

Tomography 5 Software datasheet

Preview of Tomography 5 Software datasheet

For Research Use Only. Not for use in diagnostic procedures.