Invitrogen

CD33 Monoclonal Antibody (WM-53 (WM53)), PE, eBioscience™

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CD33 Antibody (12-0338-42) in Flow

Staining of normal human peripheral blood cells with Mouse IgG1 kappa Isotype Control PE (Product # 12-4714-81) (blue histogram) or Anti-Human CD33 PE (purple histogram). Cells in the monocyte gate were used for analysis.

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产品信息

12-0338-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.25 µg)/test

查看18篇已发表文章 18篇已发表文章

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

WM-53 (WM53)

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_10855036

产品详细信息

Description: The WM-53 monoclonal antibody reacts with human CD33, also known as GP67 and P67, a 67 kDa type I transmembrane glycoprotein that is a member of the Siglec (sialic acid-binding Ig superfamily lectin) family. It is highly specific to the hematopoietic compartment and is expressed on monocytes, activated T cells, granulocytes, myeloid progenitors, and mast cells.

Applications Reported: The WM-53 (WM53) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This WM-53 (WM53) antibody has been pre-titrated and tested by flow cytometric analysis of normal human lysed whole blood. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies, and these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia. Further, CD33 is found on granulocyte and macrophage precursors in the bone marrow, but is not on pluripotent stem cells. CD33 is also expressed on, and is a useful marker for, peripheral monocytes. CD33 is useful for distinguishing myelogenous leukemia cells from lymphoid or erythroid leukemias. Diseases associated with CD43 dysfunction include gallbladder lymphoma and extracutaneous mastocytoma.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

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Fri Oct 20 00:00:00 EDT 2023

Memory-like innate response to booster vaccination with MF-59 adjuvanted influenza vaccine in children.

NPJ vaccines

Kazmin D,Clutterbuck EA,Napolitani G,Wilkins AL,Tarlton A,Thompson AJ,Montomoli E,Lapini G,Bihari S,White R,Jones C,Snape MD,Galal U,Yu LM,Rappuoli R,Del Giudice G,Pollard AJ,Pulendran B

12-0338-42 was used in Flow cytometry/Cell sorting to perform a systems-level analysis of immune responses to the trivalent inactivated influenza vaccine adjuvanted with MF-59 in children (15-24 months old) and in young, healthy adults.

Thu Jul 13 00:00:00 EDT 2023

Celiac Disease Defined by Over-Sensitivity to Gliadin Activation and Superior Antigen Presentation of Dendritic Cells.

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Hudec M,Riegerová K,Pala J,Kútna V,Černá M,O Leary VB

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Galectin-9 promotes a suppressive microenvironment in human cancer by enhancing STING degradation.

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Zhang CX,Huang DJ,Baloche V,Zhang L,Xu JX,Li BW,Zhao XR,He J,Mai HQ,Chen QY,Zhang XS,Busson P,Cui J,Li J

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Connecting METTL3 and intratumoural CD33⁺ MDSCs in predicting clinical outcome in cervical cancer.

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Ni HH,Zhang L,Huang H,Dai SQ,Li J

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iPSC-Derived Platelets Depleted of HLA Class I Are Inert to Anti-HLA Class I and Natural Killer Cell Immunity.

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Suzuki D,Flahou C,Yoshikawa N,Stirblyte I,Hayashi Y,Sawaguchi A,Akasaka M,Nakamura S,Higashi N,Xu H,Matsumoto T,Fujio K,Manz MG,Hotta A,Takizawa H,Eto K,Sugimoto N

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Tue Jan 14 00:00:00 EST 2020

Small-Molecule Targeting of Oncogenic FTO Demethylase in Acute Myeloid Leukemia.

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Huang Y,Su R,Sheng Y,Dong L,Dong Z,Xu H,Ni T,Zhang ZS,Zhang T,Li C,Han L,Zhu Z,Lian F,Wei J,Deng Q,Wang Y,Wunderlich M,Gao Z,Pan G,Zhong D,Zhou H,Zhang N,Gan J,Jiang H,Mulloy JC,Qian Z,Chen J,Yang CG

12-0338 was used in Flow cytometry/Cell sorting to show that FTO is a druggable target and that targeting FTO by small-molecule inhibitors holds potential to treat AML.

Mon Apr 15 00:00:00 EDT 2019

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Gastroenterology research and practice

Xia Y,Gao Y,Wang B,Zhang H,Zhang Q

12-0338 was used in Flow cytometry/Cell sorting to assess FACS performance of cells in bile after modification of factors that may affect cell vitality, in order to optimise the cell separation method in bile of patients with cholangiocarcinoma.

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Ultra-High-Frequency Reprogramming of Individual Long-Term Hematopoietic Stem Cells Yields Low Somatic Variant Induced Pluripotent Stem Cells.

Cell reports

Wang K,Guzman AK,Yan Z,Zhang S,Hu MY,Hamaneh MB,Yu YK,Tolu S,Zhang J,Kanavy HE,Ye K,Bartholdy B,Bouhassira EE

12-0338-42 was used in Flow Cytometry to suggest LT-HSCs may become the preferred cell source for the production of clinical-grade iPSCs.

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Surrogate in vitro activation of innate immunity synergizes with interleukin-7 to unleash rapid antigen-driven outgrowth of CD4+ and CD8+ human peripheral blood T-cells naturally recognizing MUC1, HER2/neu and other tumor-associated antigens.

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Genetic Variability Overrides the Impact of Parental Cell Type and Determines iPSC Differentiation Potential.

Stem cell reports

Kyttälä A,Moraghebi R,Valensisi C,Kettunen J,Andrus C,Pasumarthy KK,Nakanishi M,Nishimura K,Ohtaka M,Weltner J,Van Handel B,Parkkonen O,Sinisalo J,Jalanko A,Hawkins RD,Woods NB,Otonkoski T,Trokovic R

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CD19 CAR immune pressure induces B-precursor acute lymphoblastic leukaemia lineage switch exposing inherent leukaemic plasticity.

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12-0338 was used in Flow cytometry/Cell sorting to study how CD19 CAR immune pressure induces a B-precursor acute lymphoblastic leukaemia lineage switch.

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Nature medicine

Pallett LJ,Gill US,Quaglia A,Sinclair LV,Jover-Cobos M,Schurich A,Singh KP,Thomas N,Das A,Chen A,Fusai G,Bertoletti A,Cantrell DA,Kennedy PT,Davies NA,Haniffa M,Maini MK

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12-0338 was used in Flow cytometry/Cell sorting to providesproof of concept for the molecular and biological activity of novel allosteric inhibitors for targeting different mutant forms of IDH1 in leukemia.

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Functional heterogeneity of genetically defined subclones in acute myeloid leukemia.

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12-0338 was used in Flow cytometry/Cell sorting to demonstrate the importance of integrating genetic and functional data in studies of primary cancer samples.

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Targeting CDK1 promotes FLT3-activated acute myeloid leukemia differentiation through C/EBPα.

The Journal of clinical investigation

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生物信息学

蛋白别名: CD33; CD33 antigen (gp67); CD33 molecule transcript; FLJ00391; gp67; Myeloid cell surface antigen CD33; sialic acid binding Ig-like lectin 3; Sialic acid-binding Ig-like lectin 3; Siglec-3

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基因别名: CD33; p67; SIGLEC-3; SIGLEC3

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UniProt ID: (Human) P20138

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Entrez Gene ID: (Human) 945

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功能

receptor activity protein binding carbohydrate binding immunoglobulin superfamily cell adhesion molecule

参与通路

cell adhesion signal transduction cell-cell signaling negative regulation of cell proliferation regulation of immune response