Invitrogen

CD33 Monoclonal Antibody (WM-53 (WM53)), APC, eBioscience™

图: 1 / 11

CD33 Antibody (17-0338-42) in Flow

Staining of normal human peripheral blood cells with Mouse IgG1 kappa Isotype Control APC (Product # 17-4714-81) (blue histogram) or Anti-Human CD33 APC (purple histogram). Cells in the monocyte gate were used for analysis.

Product Image Gallery

产品信息

17-0338-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.125 µg)/test

查看17篇已发表文章 17篇已发表文章

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

WM-53 (WM53)

偶联物

APC APC APC

激发/发射光谱

651/660 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_10667893

产品详细信息

Description: The WM-53 monoclonal antibody reacts with human CD33, also known as GP67 and P67, a 67 kDa type I transmembrane glycoprotein that is a member of the Siglec (sialic acid-binding Ig superfamily lectin) family. It is highly specific to the hematopoietic compartment and is expressed on monocytes, activated T cells, granulocytes, myeloid progenitors, and mast cells.

Applications Reported: This WM-53 (WM53) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This WM-53 (WM53) antibody has been pre-titrated and tested by flow cytometric analysis or normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies, and these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia. Further, CD33 is found on granulocyte and macrophage precursors in the bone marrow, but is not on pluripotent stem cells. CD33 is also expressed on, and is a useful marker for, peripheral monocytes. CD33 is useful for distinguishing myelogenous leukemia cells from lymphoid or erythroid leukemias. Diseases associated with CD43 dysfunction include gallbladder lymphoma and extracutaneous mastocytoma.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Myeloid-intrinsic cell cycle-related kinase drives immunosuppression to promote tumorigenesis.

iScience

Zhou J,Wang H,Shu T,Wang J,Yang W,Li J,Ding L,Liu M,Sun H,Wong J,Lai PB,Tsang SW,Ward SE,Chow KL,Sung JJ,Sze-Lok Cheng A

Published figure using CD33 monoclonal antibody (Product # 17-0338-42) in Flow Cytometry

Fri Oct 20 00:00:00 EDT 2023

Integrated Genomic Analysis Identifies UBTF Tandem Duplications as a Recurrent Lesion in Pediatric Acute Myeloid Leukemia.

Blood cancer discovery

Umeda M,Ma J,Huang BJ,Hagiwara K,Westover T,Abdelhamed S,Barajas JM,Thomas ME,Walsh MP,Song G,Tian L,Liu Y,Chen X,Kolekar P,Tran Q,Foy SG,Maciaszek JL,Kleist AB,Leonti AR,Ju B,Easton J,Wu H,Valentine V,Valentine MB,Liu YC,Ries RE,Smith JL,Parganas E,Iacobucci I,Hiltenbrand R,Miller J,Myers JR,Rampersaud E,Rahbarinia D,Rusch M,Wu G,Inaba H,Wang YC,Alonzo TA,Downing JR,Mullighan CG,Pounds S,Babu MM,Zhang J,Rubnitz JE,Meshinchi S,Ma X,Klco JM

17-0338-42 was used in Flow cytometry/Cell sorting to define the spectrum of mutations in relapsed pediatric AML and identified UBTF-TDs as a new recurrent genetic alteration.

Thu May 05 00:00:00 EDT 2022

Ex vivo expansion of dysfunctional regulatory T lymphocytes restores suppressive function in Parkinson's disease.

NPJ Parkinson's disease

Thome AD,Atassi F,Wang J,Faridar A,Zhao W,Thonhoff JR,Beers DR,Lai EC,Appel SH

17-0338-42 was used in Flow Cytometry to document a shift towards a pro-inflammatory peripheral immune response in patients with Parkinson's disease (PD).

Thu May 13 00:00:00 EDT 2021

Multipeptide stimulated PBMCs generate T_EM/T_CM for adoptive cell therapy in multiple myeloma.

Oncotarget

Vardam-Kaur T,Pathangey LB,McCormick DJ,Bergsagel PL,Cohen PA,Gendler SJ

17-0338-42 was used in Flow Cytometry to evaluate the possibility of adoptive cell transfer (ACT) as a immunotherapeutic strategy for Multiple Myeloma (MM) patients, by generating ex vivo T cells from peripheral blood mononuclear cells (PBMCs) isolated from MM patients by employing our previously devised protocols.

Tue Sep 28 00:00:00 EDT 2021

Celiac Disease Defined by Over-Sensitivity to Gliadin Activation and Superior Antigen Presentation of Dendritic Cells.

International journal of molecular sciences

Hudec M,Riegerová K,Pala J,Kútna V,Černá M,O Leary VB

17-0338-42 was used in Flow Cytometry to identify a novel bio-profile unique to CD14++ monocytes of Celiac Disease patients.

Wed Sep 15 00:00:00 EDT 2021

Celiac Disease Defined by Over-Sensitivity to Gliadin Activation and Superior Antigen Presentation of Dendritic Cells.

International journal of molecular sciences

Hudec M,Riegerová K,Pala J,Kútna V,Černá M,O Leary VB

17-0338-42 was used in Flow Cytometry to identify a novel bio-profile unique to CD14++ monocytes of Celiac Disease patients.

Wed Sep 15 00:00:00 EDT 2021

Galectin-9 promotes a suppressive microenvironment in human cancer by enhancing STING degradation.

Oncogenesis

Zhang CX,Huang DJ,Baloche V,Zhang L,Xu JX,Li BW,Zhao XR,He J,Mai HQ,Chen QY,Zhang XS,Busson P,Cui J,Li J

Published figure using CD33 monoclonal antibody (Product # 17-0338-42) in Flow Cytometry

Mon Jul 06 00:00:00 EDT 2020

Connecting METTL3 and intratumoural CD33⁺ MDSCs in predicting clinical outcome in cervical cancer.

Journal of translational medicine

Ni HH,Zhang L,Huang H,Dai SQ,Li J

Published figure using CD33 monoclonal antibody (Product # 17-0338-42) in Flow Cytometry

Thu Oct 15 00:00:00 EDT 2020

iPSC-Derived Platelets Depleted of HLA Class I Are Inert to Anti-HLA Class I and Natural Killer Cell Immunity.

Stem cell reports

Suzuki D,Flahou C,Yoshikawa N,Stirblyte I,Hayashi Y,Sawaguchi A,Akasaka M,Nakamura S,Higashi N,Xu H,Matsumoto T,Fujio K,Manz MG,Hotta A,Takizawa H,Eto K,Sugimoto N

17-0338 was used in Flow cytometry/Cell sorting to reveal the unique non-immunogenic property of platelets.

Tue Jan 14 00:00:00 EST 2020

iPSC-Derived Platelets Depleted of HLA Class I Are Inert to Anti-HLA Class I and Natural Killer Cell Immunity.

Stem cell reports

Suzuki D,Flahou C,Yoshikawa N,Stirblyte I,Hayashi Y,Sawaguchi A,Akasaka M,Nakamura S,Higashi N,Xu H,Matsumoto T,Fujio K,Manz MG,Hotta A,Takizawa H,Eto K,Sugimoto N

17-0338 was used in Flow cytometry/Cell sorting to reveal the unique non-immunogenic property of platelets.

Tue Jan 14 00:00:00 EST 2020

Expression profiling of snoRNAs in normal hematopoiesis and AML.

Blood advances

Warner WA,Spencer DH,Trissal M,White BS,Helton N,Ley TJ,Link DC

17-0338 was used in Flow cytometry/Cell sorting to identify and quantify expression of small nucleolar RNAs in acute myeloid leukemia and normal hepatopoietic cell populations.

Tue Jan 23 00:00:00 EST 2018

Surrogate in vitro activation of innate immunity synergizes with interleukin-7 to unleash rapid antigen-driven outgrowth of CD4+ and CD8+ human peripheral blood T-cells naturally recognizing MUC1, HER2/neu and other tumor-associated antigens.

Oncotarget

Pathangey LB,McCurry DB,Gendler SJ,Dominguez AL,Gorman JE,Pathangey G,Mihalik LA,Dang Y,Disis ML,Cohen PA

Tue Feb 14 00:00:00 EST 2017

Genetic Variability Overrides the Impact of Parental Cell Type and Determines iPSC Differentiation Potential.

Stem cell reports

Kyttälä A,Moraghebi R,Valensisi C,Kettunen J,Andrus C,Pasumarthy KK,Nakanishi M,Nishimura K,Ohtaka M,Weltner J,Van Handel B,Parkkonen O,Sinisalo J,Jalanko A,Hawkins RD,Woods NB,Otonkoski T,Trokovic R

Published figure using CD33 monoclonal antibody (Product # 17-0338-42) in Flow Cytometry

Tue Feb 09 00:00:00 EST 2016

Metabolic regulation of hepatitis B immunopathology by myeloid-derived suppressor cells.

Nature medicine

Pallett LJ,Gill US,Quaglia A,Sinclair LV,Jover-Cobos M,Schurich A,Singh KP,Thomas N,Das A,Chen A,Fusai G,Bertoletti A,Cantrell DA,Kennedy PT,Davies NA,Haniffa M,Maini MK

Published figure using CD33 monoclonal antibody (Product # 17-0338-42) in Flow Cytometry

Mon Jun 01 00:00:00 EDT 2015

Defining human dendritic cell progenitors by multiparametric flow cytometry.

Nature protocols

Breton G,Lee J,Liu K,Nussenzweig MC

Published figure using CD33 monoclonal antibody (Product # 17-0338-42) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2015

Epigenomic analysis of the HOX gene loci reveals mechanisms that may control canonical expression patterns in AML and normal hematopoietic cells.

Leukemia

Spencer DH,Young MA,Lamprecht TL,Helton NM,Fulton R,O'Laughlin M,Fronick C,Magrini V,Demeter RT,Miller CA,Klco JM,Wilson RK,Ley TJ

17-0338 was used in Flow cytometry/Cell sorting to analyse RNA sequencing data from primary AML samples and normal haematopoietic cells in order to compare HOX expression in normal and leukaemic cells.

Mon Jun 01 00:00:00 EDT 2015

Functional heterogeneity of genetically defined subclones in acute myeloid leukemia.

Cancer cell

Klco JM,Spencer DH,Miller CA,Griffith M,Lamprecht TL,O'Laughlin M,Fronick C,Magrini V,Demeter RT,Fulton RS,Eades WC,Link DC,Graubert TA,Walter MJ,Mardis ER,Dipersio JF,Wilson RK,Ley TJ

17-0338 was used in Flow cytometry/Cell sorting to demonstrate the importance of integrating genetic and functional data in studies of primary cancer samples.

Mon Mar 17 00:00:00 EDT 2014

Targeting CDK1 promotes FLT3-activated acute myeloid leukemia differentiation through C/EBPα.

The Journal of clinical investigation

Radomska HS,Alberich-Jordà M,Will B,Gonzalez D,Delwel R,Tenen DG

Published figure using CD33 monoclonal antibody (Product # 17-0338-42) in Flow Cytometry

Wed Aug 01 00:00:00 EDT 2012

Characterization of a novel population of low-density granulocytes associated with disease severity in HIV-1 infection.

PloS one

Cloke T,Munder M,Taylor G,Müller I,Kropf P

Published figure using CD33 monoclonal antibody (Product # 17-0338-42) in Flow Cytometry

Wed May 01 00:00:00 EDT 2013

生物信息学

蛋白别名: CD33; CD33 antigen (gp67); CD33 molecule transcript; FLJ00391; gp67; Myeloid cell surface antigen CD33; sialic acid binding Ig-like lectin 3; Sialic acid-binding Ig-like lectin 3; Siglec-3

查看更多收起

基因别名: CD33; p67; SIGLEC-3; SIGLEC3

查看更多收起

UniProt ID: (Human) P20138

查看更多收起

Entrez Gene ID: (Human) 945

查看更多收起

功能

receptor activity protein binding carbohydrate binding immunoglobulin superfamily cell adhesion molecule

参与通路

cell adhesion signal transduction cell-cell signaling negative regulation of cell proliferation regulation of immune response