Invitrogen

CD33 Monoclonal Antibody (WM-53 (WM53)), PerCP-Cyanine5.5, eBioscience™

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CD33 Antibody (45-0338-42) in Flow

Staining of normal human peripheral blood cells with staining buffer (autofluorescence) (open histogram) or Anti-Human CD33 PerCP-Cyanine5.5 (filled histogram). Cells in the monocyte gate were used for analysis.

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产品信息

45-0338-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.5 µg)/test

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

WM-53 (WM53)

偶联物

PerCP-Cyanine5.5 PerCP-Cyanine5.5 PerCP-Cyanine5.5

激发/发射光谱

489/679 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_10714975

产品详细信息

Description: The WM-53 monoclonal antibody reacts with human CD33, also known as GP67 and P67, a 67 kDa type I transmembrane glycoprotein that is a member of the Siglec (sialic acid-binding Ig superfamily lectin) family. It is highly specific to the hematopoietic compartment and is expressed on monocytes, activated T cells, granulocytes, myeloid progenitors, and mast cells.

Applications Reported: This WM-53 (WM53) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This WM-53 (WM53) antibody has been pre-titrated and tested by fllow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 695 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies, and these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia. Further, CD33 is found on granulocyte and macrophage precursors in the bone marrow, but is not on pluripotent stem cells. CD33 is also expressed on, and is a useful marker for, peripheral monocytes. CD33 is useful for distinguishing myelogenous leukemia cells from lymphoid or erythroid leukemias. Diseases associated with CD43 dysfunction include gallbladder lymphoma and extracutaneous mastocytoma.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Myeloid-intrinsic cell cycle-related kinase drives immunosuppression to promote tumorigenesis.

iScience

Zhou J,Wang H,Shu T,Wang J,Yang W,Li J,Ding L,Liu M,Sun H,Wong J,Lai PB,Tsang SW,Ward SE,Chow KL,Sung JJ,Sze-Lok Cheng A

45-0338-42 was used in Flow cytometry/Cell sorting to demonstrated that cell cycle-related kinase (CCRK) is upregulated in myeloid cells in tumor-bearing mice and in patients with hepatocellular carcinoma.

Fri Oct 20 00:00:00 EDT 2023

Myeloid-intrinsic cell cycle-related kinase drives immunosuppression to promote tumorigenesis.

iScience

Zhou J,Wang H,Shu T,Wang J,Yang W,Li J,Ding L,Liu M,Sun H,Wong J,Lai PB,Tsang SW,Ward SE,Chow KL,Sung JJ,Sze-Lok Cheng A

Fri Oct 20 00:00:00 EDT 2023

Celiac Disease Defined by Over-Sensitivity to Gliadin Activation and Superior Antigen Presentation of Dendritic Cells.

International journal of molecular sciences

Hudec M,Riegerová K,Pala J,Kútna V,Černá M,O Leary VB

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Wed Sep 15 00:00:00 EDT 2021

Galectin-9 promotes a suppressive microenvironment in human cancer by enhancing STING degradation.

Oncogenesis

Zhang CX,Huang DJ,Baloche V,Zhang L,Xu JX,Li BW,Zhao XR,He J,Mai HQ,Chen QY,Zhang XS,Busson P,Cui J,Li J

Published figure using CD33 monoclonal antibody (Product # 45-0338-42) in Flow Cytometry

Mon Jul 06 00:00:00 EDT 2020

Connecting METTL3 and intratumoural CD33⁺ MDSCs in predicting clinical outcome in cervical cancer.

Journal of translational medicine

Ni HH,Zhang L,Huang H,Dai SQ,Li J

45-0338 was used in Flow cytometry/Cell sorting to explore the associations between the levels of methyltransferase-like 3(METTL3) and CD33+ myeloid-derived suppressor cells (MDSCs) in tumour tissues and the survival of patients with cervical cancer (CC).

Thu Oct 15 00:00:00 EDT 2020

Connecting METTL3 and intratumoural CD33⁺ MDSCs in predicting clinical outcome in cervical cancer.

Journal of translational medicine

Ni HH,Zhang L,Huang H,Dai SQ,Li J

Thu Oct 15 00:00:00 EDT 2020

iPSC-Derived Platelets Depleted of HLA Class I Are Inert to Anti-HLA Class I and Natural Killer Cell Immunity.

Stem cell reports

Suzuki D,Flahou C,Yoshikawa N,Stirblyte I,Hayashi Y,Sawaguchi A,Akasaka M,Nakamura S,Higashi N,Xu H,Matsumoto T,Fujio K,Manz MG,Hotta A,Takizawa H,Eto K,Sugimoto N

Published figure using CD33 monoclonal antibody (Product # 45-0338-42) in Flow Cytometry

Tue Jan 14 00:00:00 EST 2020

Surrogate in vitro activation of innate immunity synergizes with interleukin-7 to unleash rapid antigen-driven outgrowth of CD4+ and CD8+ human peripheral blood T-cells naturally recognizing MUC1, HER2/neu and other tumor-associated antigens.

Oncotarget

Pathangey LB,McCurry DB,Gendler SJ,Dominguez AL,Gorman JE,Pathangey G,Mihalik LA,Dang Y,Disis ML,Cohen PA

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Tue Feb 14 00:00:00 EST 2017

Genetic Variability Overrides the Impact of Parental Cell Type and Determines iPSC Differentiation Potential.

Stem cell reports

Kyttälä A,Moraghebi R,Valensisi C,Kettunen J,Andrus C,Pasumarthy KK,Nakanishi M,Nishimura K,Ohtaka M,Weltner J,Van Handel B,Parkkonen O,Sinisalo J,Jalanko A,Hawkins RD,Woods NB,Otonkoski T,Trokovic R

Published figure using CD33 monoclonal antibody (Product # 45-0338-42) in Flow Cytometry

Tue Feb 09 00:00:00 EST 2016

Metabolic regulation of hepatitis B immunopathology by myeloid-derived suppressor cells.

Nature medicine

Pallett LJ,Gill US,Quaglia A,Sinclair LV,Jover-Cobos M,Schurich A,Singh KP,Thomas N,Das A,Chen A,Fusai G,Bertoletti A,Cantrell DA,Kennedy PT,Davies NA,Haniffa M,Maini MK

Published figure using CD33 monoclonal antibody (Product # 45-0338-42) in Flow Cytometry

Mon Jun 01 00:00:00 EDT 2015

Defining human dendritic cell progenitors by multiparametric flow cytometry.

Nature protocols

Breton G,Lee J,Liu K,Nussenzweig MC

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Tue Sep 01 00:00:00 EDT 2015

Targeting CDK1 promotes FLT3-activated acute myeloid leukemia differentiation through C/EBPα.

The Journal of clinical investigation

Radomska HS,Alberich-Jordà M,Will B,Gonzalez D,Delwel R,Tenen DG

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Wed Aug 01 00:00:00 EDT 2012

Characterization of a novel population of low-density granulocytes associated with disease severity in HIV-1 infection.

PloS one

Cloke T,Munder M,Taylor G,Müller I,Kropf P

Published figure using CD33 monoclonal antibody (Product # 45-0338-42) in Flow Cytometry

Wed May 01 00:00:00 EDT 2013

Suberoylanilide hydroxamic acid reactivates HIV from latently infected cells.

The Journal of biological chemistry

Contreras X,Schweneker M,Chen CS,McCune JM,Deeks SG,Martin J,Peterlin BM

45-0338 was used in Flow cytometry/Cell sorting to demonstrate the ability of suberoylanilide hydroxamic acid to reactivate HIV from latency in chronically infected cell lines and primary cells.

Fri Mar 13 00:00:00 EDT 2009

生物信息学

蛋白别名: CD33; CD33 antigen (gp67); CD33 molecule transcript; FLJ00391; gp67; Myeloid cell surface antigen CD33; sialic acid binding Ig-like lectin 3; Sialic acid-binding Ig-like lectin 3; Siglec-3

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基因别名: CD33; p67; SIGLEC-3; SIGLEC3

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UniProt ID: (Human) P20138

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Entrez Gene ID: (Human) 945

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功能

receptor activity protein binding carbohydrate binding immunoglobulin superfamily cell adhesion molecule

参与通路

cell adhesion signal transduction cell-cell signaling negative regulation of cell proliferation regulation of immune response