Invitrogen

CD33 Monoclonal Antibody (WM-53 (WM53)), PE-Cyanine7, eBioscience™

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

10个已发表图片

21 篇参考文献

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说明书

实验方案

技术支持

说明书

实验方案

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图: 1 / 12

CD33 Antibody (25-0338-42) in Flow

Staining of normal human peripheral blood cells with Mouse IgG1 kappa Isotype Control PE-Cyanine7 (Product # 25-4714-80) (blue histogram) or Anti-Human CD33 PE-Cyanine7 (purple histogram). Cells in the monocyte gate were used for analysis.

Product Image Gallery

CD33 Antibody — Figure 8 Granulocytic differentiation of FLT3ITD cells after inhibition of CDK1 activity. ( A ) Inhibition of CDK1 in patient samples with FLT3ITD induces granulocytic differentiation. Blood specimens from FLT3ITD AML patients (patient A and patient B; same as shown in Figure Figure7) 7 ) were cultured in the presence of 0.1% DMSO or 10 mM NU6102 for 7 days. Cell aliquots were stained with anti-CD11c, anti-CD15, anti-CD14, anti-CD11b, anti-CD16, anti-G-CSF-R, anti-CD33, anti-CD133, anti-CD34, and anti-CD38 antibodies and analyzed by flow cytometry. The y axes indicate the percentage of positive cells. ( B ) Patient samples were treated as in A for 5 days and analyzed for mRNA expression of granulocytic cell-surface markers by quantitative RT-PCR. The y axes indicate relative expression to GAPDH. ( C ) CD15 + cells with granulocytic-like morphology are viable. CD15 expression on FLT3ITD AML patient A sample treated with DMSO (gray) or 5 mM NU6102 (white) during 10 days (left panel). Number indicates the percentage of CD15 + cells upon NU6102 treatment. CD15 + cells were sorted, cytocentrifuged, and stained with Wright-Giemsa method (middle panel). Original magnification, x40. CD15 + cells were also stained with annexin V and PI to determine the extent of their viability (right panel). The numbers in each quadrant indicate percentages of viable (lower left), early apoptotic (lower right), late apoptotic (upper right), and necrotic cells (upper left). {TM}

产品信息

25-0338-42

应用

建议稀释比

已发表文章

免疫细胞化学 (ICC/IF)

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流式细胞分析 (Flow)

5 µL (0.25 µg)/test

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

WM-53 (WM53)

偶联物

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

激发/发射光谱

569/780 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_1907380

产品详细信息

Description: The WM-53 monoclonal antibody reacts with human CD33, also known as GP67 and P67, a 67 kDa type I transmembrane glycoprotein that is a member of the Siglec (sialic acid-binding Ig superfamily lectin) family. It is highly specific to the hematopoietic compartment and is expressed on monocytes, activated T cells, granulocytes, myeloid progenitors, and mast cells.

Applications Reported: This WM-53 (WM53) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This WM-53 (WM53) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies, and these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia. Further, CD33 is found on granulocyte and macrophage precursors in the bone marrow, but is not on pluripotent stem cells. CD33 is also expressed on, and is a useful marker for, peripheral monocytes. CD33 is useful for distinguishing myelogenous leukemia cells from lymphoid or erythroid leukemias. Diseases associated with CD43 dysfunction include gallbladder lymphoma and extracutaneous mastocytoma.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Myeloid-intrinsic cell cycle-related kinase drives immunosuppression to promote tumorigenesis.

iScience

Zhou J,Wang H,Shu T,Wang J,Yang W,Li J,Ding L,Liu M,Sun H,Wong J,Lai PB,Tsang SW,Ward SE,Chow KL,Sung JJ,Sze-Lok Cheng A

Published figure using CD33 monoclonal antibody (Product # 25-0338-42) in Flow Cytometry

Fri Oct 20 00:00:00 EDT 2023

The ribosomal protein S6 kinase alpha-1 (RPS6KA1) induces resistance to venetoclax/azacitidine in acute myeloid leukemia.

Leukemia

Weidenauer K,Schmidt C,Rohde C,Pauli C,Blank MF,Heid D,Waclawiczek A,Corbacioglu A,Göllner S,Lotze M,Vierbaum L,Renders S,Krijgsveld J,Raffel S,Sauer T,Trumpp A,Pabst C,Müller-Tidow C,Janssen M

25-0338-42 was used in Immunocytochemistry to suggest RPS6KA1 as mediator of resistance towards venetoclax/azacitidine and additional RPS6KA1 inhibition as strategy to prevent or overcome resistance.

Tue Aug 01 00:00:00 EDT 2023

Myeloid cell subsets that express latency-associated peptide promote cancer growth by modulating T cells.

iScience

Gabriely G,Ma D,Siddiqui S,Sun L,Skillin NP,Abou-El-Hassan H,Moreira TG,Donnelly D,da Cunha AP,Fujiwara M,Walton LR,Patel A,Krishnan R,Levine SS,Healy BC,Rezende RM,Murugaiyan G,Weiner HL

25-0338-42 was used in Flow Cytometry to identify a previously unknown function by which LAPHi MCs promote tumor growth and offer therapeutic intervention to target these cells in cancer.

Fri Nov 19 00:00:00 EST 2021

Celiac Disease Defined by Over-Sensitivity to Gliadin Activation and Superior Antigen Presentation of Dendritic Cells.

International journal of molecular sciences

Hudec M,Riegerová K,Pala J,Kútna V,Černá M,O Leary VB

Published figure using CD33 monoclonal antibody (Product # 25-0338-42) in Flow Cytometry

Wed Sep 15 00:00:00 EDT 2021

Using antagonistic pleiotropy to design a chemotherapy-induced evolutionary trap to target drug resistance in cancer.

Nature genetics

Lin KH,Rutter JC,Xie A,Pardieu B,Winn ET,Bello RD,Forget A,Itzykson R,Ahn YR,Dai Z,Sobhan RT,Anderson GR,Singleton KR,Decker AE,Winter PS,Locasale JW,Crawford L,Puissant A,Wood KC

25-0338 was used in Flow cytometry/Cell sorting to perform pooled CRISPR-Cas9 knockout screens in acute myeloid leukemia (AML) cells treated with various chemotherapies to map the drug-dependent genetic basis of fitness trade-offs, a concept known as antagonistic pleiotropy (AP).

Wed Apr 01 00:00:00 EDT 2020

Galectin-9 promotes a suppressive microenvironment in human cancer by enhancing STING degradation.

Oncogenesis

Zhang CX,Huang DJ,Baloche V,Zhang L,Xu JX,Li BW,Zhao XR,He J,Mai HQ,Chen QY,Zhang XS,Busson P,Cui J,Li J

Published figure using CD33 monoclonal antibody (Product # 25-0338-42) in Flow Cytometry

Mon Jul 06 00:00:00 EDT 2020

Connecting METTL3 and intratumoural CD33⁺ MDSCs in predicting clinical outcome in cervical cancer.

Journal of translational medicine

Ni HH,Zhang L,Huang H,Dai SQ,Li J

Published figure using CD33 monoclonal antibody (Product # 25-0338-42) in Flow Cytometry

Thu Oct 15 00:00:00 EDT 2020

iPSC-Derived Platelets Depleted of HLA Class I Are Inert to Anti-HLA Class I and Natural Killer Cell Immunity.

Stem cell reports

Suzuki D,Flahou C,Yoshikawa N,Stirblyte I,Hayashi Y,Sawaguchi A,Akasaka M,Nakamura S,Higashi N,Xu H,Matsumoto T,Fujio K,Manz MG,Hotta A,Takizawa H,Eto K,Sugimoto N

Published figure using CD33 monoclonal antibody (Product # 25-0338-42) in Flow Cytometry

Tue Jan 14 00:00:00 EST 2020

Generation of Human Immunosuppressive Myeloid Cell Populations in Human Interleukin-6 Transgenic NOG Mice.

Frontiers in immunology

Hanazawa A,Ito R,Katano I,Kawai K,Goto M,Suemizu H,Kawakami Y,Ito M,Takahashi T

25-0338 was used in Flow cytometry/Cell sorting to suggest that functional human TAMs can be developed in NOG-hIL-6 Tg mice.

Thu Apr 18 00:00:00 EDT 2019

Exosomal transfer of mitochondria from airway myeloid-derived regulatory cells to T cells.

Redox biology

Hough KP,Trevor JL,Strenkowski JG,Wang Y,Chacko BK,Tousif S,Chanda D,Steele C,Antony VB,Dokland T,Ouyang X,Zhang J,Duncan SR,Thannickal VJ,Darley-Usmar VM,Deshane JS

25-0338 was used in Flow cytometry/Cell sorting to support a potential novel mechanism of cell-cell communication involving exosomal transfer of mitochondria and the bioenergetic and/or redox regulation of target cells.

Sat Sep 01 00:00:00 EDT 2018

The creatine kinase pathway is a metabolic vulnerability in EVI1-positive acute myeloid leukemia.

Nature medicine

Fenouille N,Bassil CF,Ben-Sahra I,Benajiba L,Alexe G,Ramos A,Pikman Y,Conway AS,Burgess MR,Li Q,Luciano F,Auberger P,Galinsky I,DeAngelo DJ,Stone RM,Zhang Y,Perkins AS,Shannon K,Hemann MT,Puissant A,Stegmaier K

25-0338 was used in Flow cytometry/Cell sorting to investigate the effect of MECOM on cellular metabolism.

Wed Mar 01 00:00:00 EST 2017

Surrogate in vitro activation of innate immunity synergizes with interleukin-7 to unleash rapid antigen-driven outgrowth of CD4+ and CD8+ human peripheral blood T-cells naturally recognizing MUC1, HER2/neu and other tumor-associated antigens.

Oncotarget

Pathangey LB,McCurry DB,Gendler SJ,Dominguez AL,Gorman JE,Pathangey G,Mihalik LA,Dang Y,Disis ML,Cohen PA

Published figure using CD33 monoclonal antibody (Product # 25-0338-42) in Flow Cytometry

Tue Feb 14 00:00:00 EST 2017

Antithymocyte Globulin at Clinically Relevant Concentrations Kills Leukemic Blasts.

Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation

Dabas R,Lee R,Servito MT,Dharmani-Khan P,Modi M,van Slyke T,Luider J,Durand C,Larratt L,Brandwein J,Morris D,Daly A,Khan FM,Storek J

Sun May 01 00:00:00 EDT 2016

Genetic Variability Overrides the Impact of Parental Cell Type and Determines iPSC Differentiation Potential.

Stem cell reports

Kyttälä A,Moraghebi R,Valensisi C,Kettunen J,Andrus C,Pasumarthy KK,Nakanishi M,Nishimura K,Ohtaka M,Weltner J,Van Handel B,Parkkonen O,Sinisalo J,Jalanko A,Hawkins RD,Woods NB,Otonkoski T,Trokovic R

Published figure using CD33 monoclonal antibody (Product # 25-0338-42) in Flow Cytometry

Tue Feb 09 00:00:00 EST 2016

Metabolic regulation of hepatitis B immunopathology by myeloid-derived suppressor cells.

Nature medicine

Pallett LJ,Gill US,Quaglia A,Sinclair LV,Jover-Cobos M,Schurich A,Singh KP,Thomas N,Das A,Chen A,Fusai G,Bertoletti A,Cantrell DA,Kennedy PT,Davies NA,Haniffa M,Maini MK

Published figure using CD33 monoclonal antibody (Product # 25-0338-42) in Flow Cytometry

Mon Jun 01 00:00:00 EDT 2015

Defining human dendritic cell progenitors by multiparametric flow cytometry.

Nature protocols

Breton G,Lee J,Liu K,Nussenzweig MC

Published figure using CD33 monoclonal antibody (Product # 25-0338-42) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2015

Replication of Salmonella enterica Serovar Typhimurium in Human Monocyte-Derived Macrophages.

Infection and immunity

Lathrop SK,Binder KA,Starr T,Cooper KG,Chong A,Carmody AB,Steele-Mortimer O

25-0338 was used in Flow cytometry/Cell sorting to assess the ability of Salmonella to replicate in human macrophages.

Wed Jul 01 00:00:00 EDT 2015

A novel autosomal recessive TERT T1129P mutation in a dyskeratosis congenita family leads to cellular senescence and loss of CD34+ hematopoietic stem cells not reversible by mTOR-inhibition.

Aging

Stockklausner C,Raffel S,Klermund J,Bandapalli OR,Beier F,Brümmendorf TH,Bürger F,Sauer SW,Hoffmann GF,Lorenz H,Tagliaferri L,Nowak D,Hofmann WK,Buergermeister R,Kerber C,Rausch T,Korbel JO,Luke B,Trumpp A,Kulozik AE

25-0338 was used in Flow cytometry/Cell sorting to outline limitations of a potential dyskeratosis congenita therapy based on rapamycin.

Sun Nov 01 00:00:00 EDT 2015

SYK regulates mTOR signaling in AML.

Leukemia

Carnevale J,Ross L,Puissant A,Banerji V,Stone RM,DeAngelo DJ,Ross KN,Stegmaier K

25-0338 was used in Flow cytometry/Cell sorting to study the regulation of mTOR signaling in AML by SYK.

Fri Nov 01 00:00:00 EDT 2013

Targeting CDK1 promotes FLT3-activated acute myeloid leukemia differentiation through C/EBPα.

The Journal of clinical investigation

Radomska HS,Alberich-Jordà M,Will B,Gonzalez D,Delwel R,Tenen DG

Published figure using CD33 monoclonal antibody (Product # 25-0338-42) in Flow Cytometry

Wed Aug 01 00:00:00 EDT 2012

Characterization of a novel population of low-density granulocytes associated with disease severity in HIV-1 infection.

PloS one

Cloke T,Munder M,Taylor G,Müller I,Kropf P

25-0338 was used in Flow cytometry/Cell sorting to reveal that increased frequencies of LDGs correlate with disease severity in HIV+ patients.

Wed May 01 00:00:00 EDT 2013

生物信息学

蛋白别名: CD33; CD33 antigen (gp67); CD33 molecule transcript; FLJ00391; gp67; Myeloid cell surface antigen CD33; sialic acid binding Ig-like lectin 3; Sialic acid-binding Ig-like lectin 3; Siglec-3

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基因别名: CD33; p67; SIGLEC-3; SIGLEC3

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UniProt ID: (Human) P20138

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Entrez Gene ID: (Human) 945

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功能

receptor activity protein binding carbohydrate binding immunoglobulin superfamily cell adhesion molecule

参与通路

cell adhesion signal transduction cell-cell signaling negative regulation of cell proliferation regulation of immune response