Cryo-EM University: Introduction to the Microscope

Introduction to the transmission electron microscope

Introduction to the microscope

19:58

The Hand Panels

Demonstration and introduction to the hand panels for the electron microscope, including the function of each individual knob, button and joystick.
18:31

The User Interface

Run through of the TEM User Interface, Flucam Viewer, and TEM Imaging & Analysis software.
11:50

Microscope Design

Discussion on overall microscope design and high level view of the different components.
8:43

Walk around the Microscope

Tour of the Talos Arctica Cryo-TEM.

Microscope operation control panels

9:58

Alignment and Pivot point

Principles behind the alignment and pivot point in an electron microscope.
4:50

Alignment

Walk through of the alignment control panel in the TEM User Interface and how to load alignment files.
8:05

Aperture

Walk through of the aperture control panel in the TEM User Interface.
2:37

Application Preferences

Walk through of setting the application preferences in the TEM User Interface.
9:17

Autoloader Design

Discussion of overall autoloader design and how the autoloader works.
6:33

Autoloader

Detailed walk through of the Autoloader control panel in the TEM User Interface and how to load samples.
4:24

Camera

Walk through of the camera control panel in the TEM User Interface and standard camera operations.
0:09

Control Pad

Walk through of the control pad control panel in the TEM User Interface.
11:18

Direct Alignments

Brief overview of the direct alignments available in the TEM User Interface.
1:30

Falcon Protector Calibration

Demonstration of Falcon Protector Calibration control panel in the TEM User Interface.
1:52

Falcon Protector Status

Overview of the Falcon Protector Status control panel in the TEM User Interface.
4:18

FEG Control User

Overview of the FEG Control (User) panel in the TEM User Interface for changing field emission gun settings.
4:47

FEG Registers

Overview of the FEG Registers control panel in the TEM User Interface.
0:57

Low Dose

Overview of the Low dose control panel in the TEM User Interface.
15:40

Normalization Theory

Discussion of normalization in lens hysteresis.
4:12

Normalizations

Walk through of Normalization control panel in the TEM User Interface.
9:19

Condenser Astigmatism

Discussion and review of condenser astigmatism.
6:11

Condenser Stigmation Adjustment

Walk through of the Stigmator control panel in the TEM User Interface for condenser lens stigmation adjustment.
10:53

Objective Astigmatism

Discussion on objective lens astigmatism.
9:02

Objective Stigmation Adjustment

Walk through of the Stigmator control panel in the TEM User Interface for objective stigmation adjustment.
0:53

System Status

Overview of the System Status control panel in the TEM User Interface.
11:00

Stage

Walk through of the Stage control panel in the TEM User Interface and its functions.
3:12

Temperature Control

Walk through of the Temperature Control, control panel in the TEM User Interface.
41:19

Vacuum Theory

Discussion on vacuum theory and the different vacuum pumps needed for an electron microscope.
4:14

Vacuum User

Walk through of the Vacuum (User) control panel in the TEM User Interface.
14:39

Vacuum Overview

Walk through of the Vacuum Overview control panel in the TEM User Interface.
3:37

Workspace Layout

Overview of setting up the TEM User Interface workspaces.

Talos Arctica Cryo-TEM platform cryo-holder insertion

6:59

Vacuum design on a Talos Arctica Cryo-TEM platform

Tour of the Talos Arctica Cryo-TEM vacuum system.
2:17

The retractable cryo-box

Tour of the retractable cryo-box on a Talos Arctica Cryo-TEM.
5:51

Side entry cryo-holder insertion

Demonstration of inserting a cryo-holder into the Talos Arctica Cryo-TEM.

For Research Use Only. Not for use in diagnostic procedures.