Cryo-EM University: Sample Preparation

Course directory

Sample Preparation

Preparing Hydrophilic Grids

Plunge-freezing with a Vitrobot Mark IV System

Assembling Autogrids

Loading Autogrids into Cassettes

Docking and Undocking Capsules

Course modules

Introduction to cryo-EM

Sample preparation

Introduction to the microscope

Image formation and aberrations

Condenser lens and parallel illumination

Objective, lens, stage, and projection

Detectors and cameras

Single particle analysis data collection and EPU

Entire workflow uninterrupted

Getting started in cryo-EM

Sample preparation

Preparing hydrophilic grids

Plunge-freezing with a Vitrobot Mark IV System

Assembling autogrids

Loading autogrids into cassettes

Docking and undocking capsules

Safety

Got to next module: Introduction to the microscope

For Research Use Only. Not for use in diagnostic procedures.