Cryo-EM University: Image Formation and Aberrations

Image formation and CTF

4:04

Lenses and Ray-diagrams

Overview of lenses through Ray diagrams.
7:56

How a Lens Forms an Image

General principles of how a lens forms an image.
51:39

Phase Contrast

Qualitative explanation of phase contrast.
0:00

Amplitude Contrast

Overview of amplitude contrast.
0:00

Diffraction Contrast

Theoretical overview of diffraction contrast.
0:00

Energy Filtered Contrast

Overview energy filters and concepts on how they work.

Aberrations

6:47

Spherical Aberrations

Discussion and review of spherical lens aberrations.
5:57

Chromatic Aberrations

Discussion and review of chromatic aberrations.
0:00

Coma

Review of aberrations that play a major role in transmission electron microscopes and coma.
0:00

Rotation Center and Coma

Theoretical overview of the rotation center alignment and coma.
0:00

Optimizing Rotation Center and Coma

Demonstration of optimizing rotation center and coma in the TEM User Interface.

For Research Use Only. Not for use in diagnostic procedures.

1x1 image pixel for data collection