Cryo-EM University: Single Particle Analysis Data Collection and EPU

Detailed single particle data collection

1:13:27

Data Collection

Review of the basic principles of single particle cryo-EM data collection and introduction to data acquisition.
22:08

EPU User Interface

How EPU is controlled and managed.
19:36

Setting Data Acquisition Mode in EPU

Step by step demonstration of setting the data acquisition parameters in Data Acquisition mode in EPU software.
30:19

Setting Tracking and Correction Modes in EPU

Walk through of setting tracking and correction modes in EPU software.
9:43

Calibrate Image Shifts in EPU

Demonstration of Calibrate Image Shifts routine in EPU software.
32:28

Starting Data Acquisition in EPU

Demonstration of acquiring an Atlas of your grid in EPU software.
2:30

Correcting Hole Mismatch

Demonstration of correcting hole mismatch in EPU software.
33:23

Lacey Grid Setup

Walk through of setting up a Lacey Grid in EPU software.
5:13

EPU 2.0 Autofunctions

Review and demonstration of the EPU software Auto-Functions.
21:32

EPU 2.0 Falcon Fraction Advisor

Demonstration and review of the Falcon Fraction Advisor in EPU software.
29:52

Difference between EPU Setup on Arctica vs Krios

Walk through of the differences between EPU software set up between a Talos Arctica and a Titan Krios Cryo-TEM, a 2 condenser system vs 3 condenser system.

Extensive sample screening

8:53

Cassette Loading

Walk through of settings after cassette loading in the TEM User Interface.
1:08:49

Screening a Full Cassette at Low Magnification

Demonstration of screening samples in a full cassette at low magnification in EPU software.
3:52

Record Single Atlas

Demonstration of recording a single atlas in EPU software.
22:59

Screening at High Mag and Setting up EPU

Demonstration of recording a single atlas in EPU software.
6:19

EPU 2.0 Atlas Alignment

Demonstration of setting up Altas Optics Alignment in EPU software.
4:57

C2 Aperture Alignment for Atlas

Considerations for C2 aperture alignment for Atlas.
8:49

EPU 2.0 Full Cassette Screening

Demonstration of full cassette screening in EPU software.

Krios Cryo-TEM specific EPU Software workflow

26:27

Krios Quick Microscope Check Optional

Demonstration of the checking the microscope optics on a Titan Krios Cryo-TEM.
38:13

Krios Cryo-TEM BioQuantum-K2 EPU Software setup

Demonstration of setting the parameters of EPU software for Titan Krios Bioquantum-K2.
19:03

Krios Cryo-TEM BioQuantum-K2 Sample Screening

Demonstration of setting up sample screening in EPU software for Titan Krios Cryo-TEM.
32:07

Krios Cryo-TEM BioQuantum-K2 Atlas and Data Collection

Demonstration of recording an Atlas and data collection in EPU software.
6:08

Krios C2 Atlas Alignment

Demonstration of C2 Atlas alignment in a three condenser system.

Basic Titan/Krios Cryo-TEM performance check

0:00

Titan Krios Cryo-TEM Performance Check

Walk through of performance checks for a Titan Krios Cryo-TEM.

For Research Use Only. Not for use in diagnostic procedures.