Cryo-EM University: Objective Lens, Stage, and Projection

Objective lens, focus, and eucentric height

23:10

The Objective Lens

Theoretical overview of the function of the objective lens in an electron microscope.
20:29

Focus

Explanation and review of when a lens is in focus.
6:54

Eucenctric Height

The importance of eucentric height.
17:42

How to set Eucentric Height

Demonstration of how to set the Eucentric height in the TEM User Interface.
49:10

Different Ways to Determine Focus

Theoretical explanation on the different ways to focus.
32:29

How to Focus on an Electron Microscope

Demonstration on how to focus the electron microscope in the TEM User Interface.

Projection

43:58

Projection and Magnification

Explanation of what is magnification and projection in an electron microscope.
24:40

Changing Magnification on an Electron Microscope

Demonstration on how to change magnification on an electron microscope through the TEM User Interface.
2:44

Camera Length

Demonstration of the changing camera length to change the magnification.

Effect of not being parallel on the image

11:09

Consequences of Not being Parallel

Overview and explanation of the consequences of when you are not parallel illuminating your specimen.
9:23

Imaging while Not being Parallel

Demonstration of the consequences of not parallel illuminating your specimen in the TEM User Interface.

Apertures and astigmatism

25:27

The Objective Aperture

Overview of the objective aperture and its function.
6:33

How to Align the Objective Aperture

Demonstration on how to align the objective aperture in the TEM User Interface.
6:04

Contrast Enhancement using the Objective Aperture

Demonstration of the contrast enhancement using the objective aperture in the TEM User Interface.
10:53

Objective Astigmatism

Overview and explanation of the objective lens astigmatism.
0:00

How to Correct Objective Astigmatism

Demonstration on how to set objective stigmation through the TEM User Interface.
0:00

Diffraction Astigmatism

Theoretical explanation on diffraction astigmatism, which comes from the diffraction lens.
0:00

When to Correct Diffraction Astigmatism

Demonstration of when to correct diffraction astigmatism in the TEM User Interface.

For Research Use Only. Not for use in diagnostic procedures.