Getting started in cryo-EM

Sample preparation for single particle cryo-EM

8:12

Discoveries in structural biology, biochemistry, and cell biology

Cryo-EM provides valuable research solutions in structural biology, biochemistry, biophysics, and cell biology. See some of the research impacted by cryo-EM.
10:33

Workflow Considerations for Sample Preparation for Cryo-EM

Important considerations for sample preparation for single particle cryo-EM.
10:03

Instrumentation and infrastructure for the single particle cryo-EM analysis

Specific considerations for infrastructure, lab and personnel requirements for single particle analysis.

Microscope operations for cryo-EM

14:48

Cryo-EM grid screening strategies

General considerations for grid screening before data collection for single particle cryo-EM.
10:26

Cryo-EM data collection strategies

Suggestions for data collection strategies and how to get the most from data collection.
36:46

Tundra Cryo-TEM virtual demonstration

In this virtual demonstration, see the Thermo Scientific Tundra Cryo-TEM in action from sample preparation to data collection.

Single particle cryo-EM data analysis

7:15

Assessing cryo-EM image and particle quality

Important considerations that should be made before data processing begins.
9:49

Cryo-EM data processing workflow

Typical steps of a standard single particle cryo-EM workflow.
8:39

Assessing quality of 3D reconstructions

General considerations to keep in mind after making a 3D reconstruction.

Basics of data processing for single particle cryo-EM

0:00

Overview of 3D reconstruction for single particle cryo-EM

Basics of data analysis and reconstruction for single particle analysis- general overview of the steps needed to generating a 3D reconstruction.
0:00

Resolving structural heterogeneity

Basics of data analysis and reconstruction for single particle analysis- resolving structural heterogeneity.

For Research Use Only. Not for use in diagnostic procedures.

1x1 image pixel for data collection