3.31截止!胰酶/Lipo3000/血清等开工特惠, 2025首单/满额加赠积分,积分可兑Gibco定制冰箱贴
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Introduction to cryo-EM
Sample preparation
Introduction to the microscope
Image formation and aberrations
Accelerator
Condenser lens and parallel illumination
Objective, lens, stage, and projection
Detectors and cameras
Single particle analysis data collection and EPU
Entire workflow uninterrupted
Getting started in cryo-EM
Demonstration and introduction to the hand panels for the electron microscope, including the function of each individual knob, button and joystick.
Run through of the TEM User Interface, Flucam Viewer, and TEM Imaging & Analysis software.
Discussion on overall microscope design and high level view of the different components.
Tour of the Talos Arctica Cryo-TEM.
Principles behind the alignment and pivot point in an electron microscope.
Walk through of the alignment control panel in the TEM User Interface and how to load alignment files.
Walk through of the aperture control panel in the TEM User Interface.
Walk through of setting the application preferences in the TEM User Interface.
Discussion of overall autoloader design and how the autoloader works.
Detailed walk through of the Autoloader control panel in the TEM User Interface and how to load samples.
Walk through of the camera control panel in the TEM User Interface and standard camera operations.
Walk through of the control pad control panel in the TEM User Interface.
Brief overview of the direct alignments available in the TEM User Interface.
Demonstration of Falcon Protector Calibration control panel in the TEM User Interface.
Overview of the Falcon Protector Status control panel in the TEM User Interface.
Overview of the FEG Control (User) panel in the TEM User Interface for changing field emission gun settings.
Overview of the FEG Registers control panel in the TEM User Interface.
Overview of the Low dose control panel in the TEM User Interface.
Discussion of normalization in lens hysteresis.
Walk through of Normalization control panel in the TEM User Interface.
Discussion and review of condenser astigmatism.
Walk through of the Stigmator control panel in the TEM User Interface for condenser lens stigmation adjustment.
Discussion on objective lens astigmatism.
Walk through of the Stigmator control panel in the TEM User Interface for objective stigmation adjustment.
Overview of the System Status control panel in the TEM User Interface.
Walk through of the Stage control panel in the TEM User Interface and its functions.
Walk through of the Temperature Control, control panel in the TEM User Interface.
Discussion on vacuum theory and the different vacuum pumps needed for an electron microscope.
Walk through of the Vacuum (User) control panel in the TEM User Interface.
Walk through of the Vacuum Overview control panel in the TEM User Interface.
Overview of setting up the TEM User Interface workspaces.
Tour of the Talos Arctica Cryo-TEM vacuum system.
Tour of the retractable cryo-box on a Talos Arctica Cryo-TEM.
Demonstration of inserting a cryo-holder into the Talos Arctica Cryo-TEM.
For Research Use Only. Not for use in diagnostic procedures.