Cryo-EM University: Condenser Lens and Parallel Illumination

Two condenser system

8:36

Condenser Lens 1 - Spotsize

Theoretical review of the first and second condenser lens within the electron microscope and what happens when you change the spot size.
22:56

Condenser Lens 2 - Intensity

Review of the second condenser lens and the function of the aperture to change the dose rate or flux.
9:13

Parallel Illumination

Overview of parallel illumination of the specimen.
10:48

Intensity Change

Theoretical review of how beam intensity is changed on the specimen in a two condenser system.

Three condenser system

11:05

2 Condenser vs. 3 Condenser

Discussion of the difference between two condenser lens systems and a three condenser lens systems.
33:39

Microprobe vs. Nanoprobe

Review of the difference between TEM and probe mode, and overview of microprobe and nanoprobe mode within TEM mode.
13:18

Beam Size at Parallel Illumination

Discussion of the differences between parallel illumination in a 2 condenser lens and 3 condenser lens system on beam size.

Two condenser system operation

31:12

Two condenser system operation

Demonstration of the operation of a two condenser system.

33:32

Parallel illumination with two condenser system

Demonstration on how to set parallel illumination in a two condenser system.

9:19

Condenser astigmatism

Discussion and review of condenser astigmatism.

6:11

Stigmators

Demonstration and review of the Stigmator control panel in the TEM User Interface to change the condenser or objective stigmator.

Three condenser system operation

44:47

3 Condenser System Operation

Demonstration of the operation of a three condenser lens system.
3:31

Condenser Astigmatism in 3 Condenser Mode

Demonstration of the condenser astigmatism in three condenser lens mode.

Aperture and zoom alignment

13:31

Condenser Aperture

Theoretical review of the C2 aperture and its alignment.
4:07

How to Align a C2 Aperture

Step by step demonstration on how to align the C2 aperture in the TEM User Interface.
14:06

C2 Apertures on a 3 Condenser

Demonstration of alignment the C2 aperture on a three condenser lens systems.

Fringes and STEM

29:37

STEM

Theoretical review of the difference between TEM and STEM.
27:22

Fresnel Fringes

Theoretical review of the source of C2 Fresnel fringes.
9:26

Practical Consequences of Fresnel Fringes on Titan Krios Cryo-TEM

Walk through of effects of C2 fringes on three condenser lens systems, such as the Titan Krios Cryo-TEM.
8:01

Practical Consequences of Fringes on Talos Arctica and Glacios Cryo-TEM

Walk through the effects of C2 fringes on two condenser lens systems, such as the Talos Arctica and Glacios Cryo-TEM.

For Research Use Only. Not for use in diagnostic procedures.

1x1 image pixel for data collection